A sensitive, semi-quantitative mammalian two-hybrid assay
نویسندگان
چکیده
منابع مشابه
Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
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Galactose metabolism Galactose is imported into the cell and converted to galactose-6-phosphate by six enzymes (GAL1, GAL2, PGM2, GAL7, GAL10, MEL1) which are transcriptionally regulated by the proteins Gal80, Gal3, and Gal4, the latter of which plays the central role of DNA-binding transactivator (Figure 1). Gal80 binds Gal4 and inhibits its transcriptional ability. Gal3, in the presence of ga...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2017
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114544